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Image Search Results
Journal: Arthritis Research & Therapy
Article Title: Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes
doi: 10.1186/ar3758
Figure Lengend Snippet: Expression of Notch receptors and ligands during osteoclastogenesis from mouse BM . BM cells cultured with rmM-CSF for 72 hours were used as osteoclast precursors (Day 0). These cells were further cultured with rmM-CSF and rhRANKL to induce osteoclasts. ( a ) CD11b + F4/80 + osteoclast precursors and CD11b + CD61 + osteoclasts were analyzed on days 0, 3 and 5. Percentages from three independent experiments are indicated. ( b ) Expression of Notch receptors and ligands on CD11b + F4/80 + osteoclast precursors was analyzed by flow cytometry on days 0, 3 and 5. Expression on CD11b + F4/80 - cells on Day 3 is also represented. CD11b + CD61 + osteoclasts were analyzed on Day 5. For comparison, BM cells were cultured with rmM-CSF alone for eight days and CD11b + F4/80 + cells were analyzed as BM-derived macrophages (BMMø), or with rmGM-CSF for eight days with LPS stimulation for the last 17 hours and CD11c + cells were analyzed as BM-derived dendritic cells (BMDC). Filled histograms indicate the control hamster IgG. Open histograms indicate the Notch receptors or ligands. ( c ) Messenger RNA levels of Notch ligands were measured by RT-PCR on days 0, 3 and 5.
Article Snippet: These cells (1 × 10 5 cells/ml) were cultured in the presence of 20 ng/ml
Techniques: Expressing, Cell Culture, Flow Cytometry, Derivative Assay, Reverse Transcription Polymerase Chain Reaction